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KMID : 1189420220350020001
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2022 Volume.35 No. 2 p.1 ~ p.12
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Effects of Aster glehni Water Extracts on Skin Inflammation in HaCaT Keratinocytes
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Kim Se-Jung
Lee Kyou-Young
Kim Geun-Lip
Hong Chul-Hee
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Abstract
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Objectives : This study was conducted to verify the anti-inflammatory effects of Aster glehni water extracts in HaCaT keratinocytes.
Methods : In this study, cell viability was confirmed by MTT assay. Production of TNF-α and IL-6 was determined by ELISA. mRNA expression of TARC and MDC were measusred by qRT-PCR. Also, expressions of p-JNK, JNK, p-ERK, ERK, p-p38, and p-38 were investigated by using western blot assay.
Results : Aster glehni water extracts were not shown any significant cytotoxicity at 15.625-500㎍/㎖ in HaCaT keratinocytes. Aster glehni extracts inhibited the TNF-α and IL-6 production in HaCaT keratinocytes treated with TNF-α and IFN-γ. Also, expression of TARC, MDC, p-ERK, and p-STAT1 was decreased.
Conclusions : These results suggest that Aster glehni water extracts have anti-inflammatory effects in HaCaT keratinocytes and can be applied to the development of anti-inflammatory treatment substances.
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KEYWORD
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Aster glehni, Anti inflammation, HaCaT keratinocytes, TNF-α, IL-6
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